Project Deliverables

Specific Aim I: Delimit homeologous regions of the soybean genome by developing an integrated genetic-physical map.

• 2,750 ESTs to be placed on the physical map via overgos
  • 2,000 at Purdue Univ. (1,000 yr 1 and 1000 in yrs 2-3)
  • 750 at Iowa State Univ. in yrs 1-2
• Placing SSRs/STSs on physical map via BAC pools at Missouri
  • 750 SSRs/STS screened against BAC pools in yr 1
  • 750 SSRs/STS screened against BAC pools in yr 2
• FISH Mapping at Purdue University
  • EST rich and EST poor BACs in yrs 1-3
  • Integrate linkage groups yrs 1-3
• STC database
  • 225,000 BES at Univ. Arizona in yr 1
  • 275,000 FES at Univ. Arizona in yr 2
• Contig/BAC anchoring to genetic map via SSRs (Iowa State Univ.)
  • Develop/test 1,200 and map ~500 yrs 1-3
• EST mapping and targeting of homeologous BACs for sequencing at Iowa State Univ.

Specific Aim II: Examine structural evolution of duplicated regions by sequencing EST rich and EST-poor BACs and homeologous BACs.

• 40 BACs to Phase III at Univ. of Arizona
  • 10 in yr 1 selected by EST density
  • 30 in yr 2 selected by EST density
• 64 BACs to Phase II at Purdue University
  • 4 in yr 1 selected by low EST density
  • 50 in yr 2-3 paralogous to BACs sequenced at Univ. Arizona

Informatics

• LIS at NCGR: 1/2 a person to do bimestrial uploads of SoyMap data into LIS
• Purdue: 1 full-time person to:
  • maintain project website
  • central clearinghouse for project data
  • prepare data for LIS

Table Summary of Project Deliverables

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