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SOYMAP SUMMER RESEARCH PROGRAM 2016
     

GRAHAM LAB AT IOWA STATE UNIVERSITY

The laboratory is located on the Iowa State University campus and conducts structural and functional analyses of the soybean genome. The lab works closely with the National Center for Genomic Resources in Santa Fe, NM. As part of the National Science Foundation project, a summer intern will have several choices of projects in which to become involved. In each case the intern will learn routine molecular genetic and computer techniques and will be taught how to apply them in a team laboratory setting.

The intern will begin by analyzing DNA sequence that has been generated from clones used in the primary construction of a physical map of soybean chromosomes.

   

Two potential projects include:

  • PROJECT 1:

    The intern will be taught how to use the DNA sequence to develop genetic markers that will help to locate the clone to specific regions on the physical map. The intern will use sequences from unknown locations to identify the clones to which they belong. Also, the intern will be given several groups of clones thought to belong together at the same location and will use laboratory techniques to confirm their location. Some clones will remain with the group, and some will be tossed out. If time permits the intern may determine the correct group in which to place the clones.

  • PROJECT 2:

    The intern will be taught how to use the DNA sequence to develop genetic markers that will help to locate the clone to specific regions on the chromosome through a genetic `road map'. The intern will use computer programs to identify simple DNA sequence repeat units called 'SSRs'. The intern will be taught how to develop laboratory tools that will permit them to follow the SSR through soybean generations. Using existing soybean mapping populations the intern will collect segregation data, enter it into a computer program, and genetically map the SSR. This process will anchor the clones to the physical map, and to the genetic map.

In each case the intern will be taught everything they need to know to complete the project. They will work closely with Graduate Students, Technicians, senior-level PhDs in the lab, and often with other Iowa State undergraduates. The student intern will have an opportunity to participate in group meetings, attend University seminars, and to participate in a research project of national importance.

   

University of Georgia USDA Agricultural Research Service Iowa State University National Science Foundation